动脉粥样硬化敏感小鼠C_（57）BL／6J腹膜巨噬细胞源性泡沫细胞模型的建立

Founding of a Model of Macrophage-derived Foam Cell from Peritoneum in C_57BL/6J Mouse which Is Susceptible to Atherosclerosis

泡沫细胞的出现是动脉粥样硬化斑块中有特征性的病理形态改变。本实验选择对动脉粥样硬化形成较敏感的纯系小鼠Ｃ５７ＢＬ／６Ｊ，取其腹膜巨噬细胞与１０ｍｇ·Ｌ－１氧化低密度脂蛋白共同孵育９６ｈ，建立了典型的巨噬细胞源性泡沫细胞模型。实验结果显示，培养的泡沫样细胞与在无脂蛋白培养基中培养的Ｃ５７ＢＬ／６Ｊ小鼠巨噬细胞和在氧化低密度脂蛋白中培养的昆明种小鼠巨噬细胞比较，脂质成分大量增多，出现脂质颗粒，细胞内总胆固醇增加，其中胆固醇酯含量大于游离胆固醇，符合泡沫细胞的定义。本实验从离休细胞培养方面探寻建立小鼠巨噬细胞源性泡沫细胞的可能性，为动脉粥样硬化的形成机理分析和防治研究提供了一种可靠的病理细胞模型。

In this study, a typical model of macrophages-derived foam cell was founded by peritoneal macrophage of C57 BL/6J mouse, which is susceptible to atherosclerosis, incubated with 10 mg·L-1 oxidized low density lipoprotein. Comparing with macrophages of Swiss strain mouse, these results showed the cultured foam cell-derived from C57 BL/6J mouse macrophages continued a large of lipid particles and intracellular total cholesterol increased in the cells, in which cholesteryl ester occupied more than 50% of total cholesterol. It corresponded to the typicalcharacteristics of foam cells. This model suppled a reliable pathological cell tool for the study of atherosclerosis.