温州蜜柑船形叶病因研究再报

Citrus Tristeza Virus (CTV)Associated with the Boat-shape-Ieaf Symptom of Satsuma Mandarin

试验表明我国田间温州蜜柑上的船形叶多数不是由温州蜜柑萎缩病毒（ＳＤＶ）所致，其中部分具有嫁接传染性，经ＥＬＩＳＡ检测带柑桔衰退病毒（ＣＴＶ）。用ＣＴＶ接种温州蜜柑，可致船形叶症状。推测我国田间温州蜜柑船形叶症状主要系ＣＴＶ所致。

Boat-shape-leaf is commonly appeared in satsuma orchards in China。 This symp-tom is usually thought to be associated with satsuma dwarf virus(SDV).Since 1985,80Samples of satsuma with boat-shape-leaf collected from 9 provinces were identified for SDVinfection by indicator, white sesame (Sesamum indicum ) and by ELISA。The results showedthat 3 out of 80 samples,which collected from the plants imported from Japan in 1980’s,wereinfected with SDV,the others are SDV-free.Boat-shape-leaf of some of the SDV-free sam-ples are graft-transmissible. All of 18 SDV-free samples detected by ELISA with CTV anti-serum were found to be infected with CTV. More severe bOat-shape-leaf symptom could beinduced by heavy pruning of the tree in spring。The resuIts of graft-inoculating CTV intovirus-free satsuma showed that CTV could also cause boat-shape-leaf symptcm。It is sug-gested that boat-shape-leaf symptom of satsuma in China are associated with CTV in gene-ral。