Dot－ELISA检测抗原与IHA、COPT检测抗体诊断血吸虫病的比较

COMPARISON OF DOT-ELISA FOR DETECTING CIRCULATING ANTIGEN WITH IHA, COPT FOR DETECTING ANTIBODY FROM SCHISTOSOMIASIS PATIENT

本文比较了斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）检测循环抗原，间接血凝试验（ＩＨＡ）、环卵沉淀试验（ＣＯＰＴ）检测抗体诊断血吸虫病的效果。１２例急性血吸虫病患者血清３种方法检测均为阳性；２７７例慢性血吸虫病患者血清３种方法检测阳性率分别为７９．４２％、６４．６２％和６３．１８％。１４例华支睾吸虫病患者血清和８９例健康人血清Ｄｏｔ－ＥＬＩＳＡ检测均为阴性。１０例急性血吸虫病患者，在离开流行区经吡喹酮治疗后３７周时粪检全部转阴，检测血清循环抗原４例转阴，其余６例滴度大幅度下降；ＩＨＡ检测３例转阴，其余７例几何平均倒数滴度（ＧＭＲＴ）由４７．５７降至９．３５；ＣＯＰＴ检测平均环沉率由２１．５％降至４．１２％。结果表明Ｄｏｔ－ＥＬＩＳＡ检测血吸虫循环抗原具有较好的敏感性和特异性。这３种方法在血吸虫病诊断及疗效考核中均有一定价值。

The comparison of Dot- ELISA used for detecting circulating antigen with IHA,COPT trials for detecting antibody were made on schistosomiasis patient. With the three methodsof Dot- ELISA, IHA and COPT,all 12 sera from acute schistosomiasis patients were positive,while the positive rate of277 sera from chronic schstosomiasis patients by the three methodswere 79. 42%, 66and6 18%respectively. The positive rate of Dot-ELISA for detectingcirculatingantigen was higher than of IHA and COPT for detecting antibody(P<0. 01 ).All 14sera from Clonorchis sinensis patients and 89 normal sera were negative by Dot- ELISA,4 serafrom 10acute schistosomiasis patients, who were negative with stool examination after leavingendemic area and 37 weeks after treatment with praziquantel, became negative with Dot- ELISA : In the other 6 cases GMRT declined from 705. 02 before treatment to 4. 92 after treat-ment; Only 3 cases were negative by IHA and GMRT of other 7 cases came down from 47. 57 be-fore treatment to 9. 35 after treatment ; 4 cases were detected with COPT and the rates of circum-oval precipitin were below 3%, the circumoval precipitin rates of 2 cases were 1%; Average pre-cipitin rate decreased from 21.5%before treatment to 4. 12%after treatment. The results of Dot- ELISA showed higher sensitivity and specificity for the detection of circulating antigen. Dot- ELISA, COPT and IHA were of value for effective evaluation of schistosomiasis treatment.