高温、CO及其联合作用对大鼠HSP70mRNA，HSP70合成影响研究

Combined Effects of High Temper-ature and Carbon Monoxide on Rat Liver HSP70mRNA and its Synthesis

当组织细胞对热或其它刺激反应时，均被诱导增加ＨＳＰｓ合成。本研究将８只雄性大鼠随机分为四组，分别为对照组、高温组、ＣＯ组、高温＋ＣＯ组。在送氧式高温与毒物联合作用装置中染毒１ｈ，在室温下恢复１ｈ后，测定其肛温、ＨｂＣＯ。断头处死，取其肝脏分析ＨＳＰ７０ｍＲＮＡ和ＨＳＰ７０。结果如下：（１）与对照组相比，高温组、高温＋ＣＯ组大鼠肛温均升高３．０℃以上，ＣＯ组及高温＋ＣＯ组大鼠ＨｂＣＯ含量均达３０％以上，这说明受热及染毒后动物的体温及ＨｂＣＯ含量均符合本实验要求。（２）结果显示，对照组仅有少量ＨＳＰ７０ｍＲＮＡ，与之相比，ＣＯ组有所增加，高温组增加明显，而高温＋ＣＯ组增加更为明显，表现为协同作用。ＨＳＰ７０合成也与ＨＳＰ７０ｍＲＮＡ结果一致。

he HSPs(heat stress proteins)could beinduced by heat or other stimuli,the normalproteins was inhibited. Present study aimedat the effects of high temperature,carbonmonoxide and their combined effects on the major heat stress protein-HSP70 and its mRN-A.8 male rats were randomly divided intofour groups: control group(25.0+1.0℃, hightemperature group(40.0 + 2.0℃),carbon mon-oxide group(600+50ppm, 25.0+1.0℃)and thecombine effects group(600+50ppm, 40+2.0℃).After experiment, the rats rceovered in roomtemperature for 60 min. Then determined therectal temperature and HbCO in the blood,the liver were taken for HSP70 and its mR-NA for analysis. The results were as follws:(1)the rectal temperature in high tempera-ture group and combined effect group, show-ed a 3. 0℃;HbCO level in CO group andcombined effect group increased above 30%,respectively compared with control group.(2)only little HSP70 mRNA was transcripted in control group, showed little increase HSP70mRNA was obviously increased in high temp-erature group, and the increase of HSP70mRNA in combined effect group was most obvious, suggesting a synergistic action betw-een high temperature and CO. The synthesisof HSP70 showed a consistent changes withthat of its mRNA in all groups.