摘要
连续９０天饮用含甲基汞浓度为１／１０００ＬＤ５０、１／１００ＬＤ５０、１／５０ＬＤ５０和１／１０ＬＤ５０的自来水的雄性昆明小鼠，各脏器中总汞含量均高于对照组（Ｐ＜０．０５～０．００５），并且随着染毒剂量增加，脏器中总汞含量也随之增高。同时采用ＦＡＣＳｃａｎ流式细胞仪和“ＣｅｌｌＦＩＴ”软件分析脾细胞周期进程，发现除１／１０００ＬＤ５０剂量组外，其余各剂量组从Ｇｏ／Ｇ１时相进入Ｓ时相的脾细胞百分数均明显高于对照组（Ｐ＜０．０５），与染毒剂量呈明显正相关。表明连续经口摄入低剂量甲基汞小鼠脾细胞周期进程加快，细胞ＤＮＡ复制增强。
Male Kunming mice drank continuously tap water containing methylmercury with the concentration of 1/IOOO、1/100、1/50、1/10 LD50 for 90 days. For all groups. mercury in each internal organ was more than that of control (P<0. 05～0. 005 ), and it increased with the increase of contaminative dose. At the same time, the cell cycle progression of splenocytes was analyzed by a FACS can flow cytometry with software 'CELLFIT'. It was found that the percentage of splenocytes with S phase from G0/G1 phase in those groups except 1/1000 LD50 group was significantly higher than in control (P <0. 05 ), correlated positively with contaminative dose. The results suggest that the cell cycle progression and DNA replication of splenocytes in mice were enhanced significantly by low dose methylmercury intaked contin continuously via mouth.
基金
国家自然科学基金
关键词
低剂量
甲基汞
脏器汞含量
细胞周期进程
Low dose methylmereury Mercury in intern organ Cell cycle progression
