摘要
采用光镜免疫组织化学结合计算机图像定量分析研究了3种食物蛋白质对幼鼠中央杏仁核生长抑制素(SOM)神经元生长发育的作用.结果表明:全蛋、鱼肉蛋白组幼鼠中央杏仁核SOM神经元单位体积粒子密度和胞体和面积均明显高于全谷组(P<0.05),而细胞灰度值则以全谷组为高(P<0.05),显示了动物蛋白质在体内有很强的促进幼鼠脑神经元生长发育的作用;从形态学角度为研究优质蛋白质促进脑生长作用机理提供了证据.
The effects of different type of dietary proteins on the development of somatostatin (SOM)neurons in the central amygdala were observed in weanling rats fed diet with either whole egg, carp fish or corn + glutin (CP). Demonstrated by ABCABC immunohistochemical double stain method, following the imaging quantitative analysis, the result showed that both the SOM neuron number of per unit volume and the average area of cross section of the neuron were lower, but the gray value of the SOM neurons were significantly higher in CP group.than in the other groups. The study suggested that types of dietary protiens play an important role in early development of SOM neurons.
关键词
食物蛋白质
中央杏仁核
生长抑制素
免疫组化
Dietary protein Central amygdala Somatostatin Immunohistochemistry Image quantitative analysis