创面愈合中外用中药对免疫活性细胞氧化代谢功能的影响──偎脓长肉作用机制研究之三

Influence of immuno-activity of cellular oxidation metabolic function of the external used Chinese herb during wound healing-Study on the mechanism of Wei Nong Zhang Rou(3)

本文采用化学发光技术（Ｃｈｅｍｉｌｕｍｉｎｅｓｅｎｃｅ－ＣＬ），检测外用中药在创伤愈合中对中性粒细胞（ＰＭＮ）、淋巴细胞（ｌｙ）、创面渗出细胞的氧化代谢功能，以及创面渗出液对正常ＰＭＮ和淋巴细胞－ＣＬ的影响。实验结果表明，外用中药可激活ＰＭＮ和淋巴细胞产生－ＣＬ；与对照组比较呈极显著差异（Ｐ＜０．０１）。在创面愈合中ＰＭＮ率先活化而后激活淋巴细胞持续到愈合期。创面渗出细胞有较强的－ＣＬ活性，外用中药组优于对照组（Ｐ＜０．０１）。外用中药组创面渗出液可作为ＰＭＮ和淋巴细胞的激活因子，具有与酵母聚糖（Ｚｙｍｏｓａｎ）和刀豆球蛋白－Ａ（ＣｏｎＡ）雷同的作用，而对照组则作用极弱。

Oxidation metabolic function of neutrophils ,lymphocytes,exudate cells of wound surface and the influence of wound surface exudation on normal neutrophils and lumphocytes were detected by means of chemiluminescence-Cl during application of Chinese herbs on wound healing.The results of experiments indicated that external applying of Chinese herbs can activate neutrophils and lymphocytes to produce-Cl. The difference is very evident (P<0.01) as compared with control group. During wound healing neutrophils were activated at first then lymphocytes till healing stage. The exudation cells of the wound surface bears rather strong-Cl activity. Those of the external used Chinese herb group is superior than control group (P<0.01 ).Exudation of external applied Chinese herb group can serve as activator of neutrophils and lymphocytes ,it bears similar action as zymosan and canavaline A.There is very weak action in control group.