异盘和卫氏、扁囊并殖吸虫MDH、EST、POD同工酶的比较研究

COMPARATIVE STUDIES ON MDH、EST、POD ISOENZYME PATTEKNS OF PARAGONIMUS HETEROTREMUS,P.WESTERMANI AND P.ASYMMETRICUS

本研究采用等电点聚焦电泳方法对异盘、卫氏、扁囊３种并殖吸虫的ＭＤＨ、ＥＳＴ、ＰＯＤ３种同工酶进行检测，结果显示卫氏和扁囊并殖吸虫３种同工酶的酶带数目及等电点分布完全一致，证明卫氏与扁囊并殖吸虫为同一虫种。异盘、卫氏和扁囊并殖吸虫ＥＳＴ同工酶酶带均为１０条，其中等电点分布相同的５条，不同的５条。异盘与卫氏、扁囊并殖吸虫分别有１０条和７条ＭＤＨ同工酶酶带，这３种并殖吸虫共同的有３条酶带。异盘与卫氏、扁囊并殖吸虫分别有７条和１６条ＰＯＤ同工酶酶带，其中仅１条酶带为３种并殖吸虫所共有。结果表明异盘并殖吸虫为一独立虫种。

The malate dehydrogenase(MDH ),esterase( EST ),and peroxidase(POD)isoenzyme patterns of Paragonimus heterotremus(h),P. westermani(w)and P. asymmetricus(a)were determined by isoelectric focusing(IEF) in polyacrylamide gel. Results showed that the number of isoenzyme bands and the isoelectric point distributions(IPD)of P.w. and P.a. were completely in agreement.Therefore,P. w and P. a were identical species.P.h,P.w,and P.a. all had 10 EST isoenzyme bands,among them, 5 bands were identical, 5 bands were different between P. w and P. a at IPD.P.h. had 10 MDH isoezyme bands,P.w. and P.a. had 7 identical MDH isoenzyme bands respectively. There were 3 MDH isoenzyme bands with identical IPO in P.h,P.w.and P.a.,Either of P.w,and P.a.had 16 POD isoenzyme bands,P.h. had only 7. Only one POD isoenzyme band was identical at IPD in all the three species. The results demonstrated that P.h. was an independent species of Paragonimus.