摘要
将受检者血清免疫复合物（ＣＩＣ）用改进方法解离后，以多克隆、单克隆抗体（ＰｃＡｂ、ＭｃＡｂ）及可溶性血吸虫卵抗原（ＳＥＡ）作ＥＬＩＳＡ检测血吸虫特异性抗原（Ａｇ）及抗体（Ａｂ）。结果表明，检测ＣＩＣ解离后的Ａｇ（称为非游离性循环抗原，ＮＦ一ＣＡｇ）和Ａｂ（称为非游离性循环抗体，ＮＦ一ＣＡｂ），均显示满意的特异性和敏感性；用抗ＳＥＡ一ＰｃＡｂ与抗ＳＥＡ一ＭｃＡｂ和抗ＣＣＡ一ＭｃＡｂ对比检测ＮＦ一ＣＡｇ时，显示相似的检测效果；该法具有诊断病人和考核治疗效果的价值，且有互补诊断血吸虫病的效果。血清中ＣＩＣ解离后检测血吸虫特异性的ＮＦ一ＣＡｇ／ＮＦ一ＣＡｂ可望成为血吸虫病血清学诊断的新途径。
Detection of Schistosome specific
antigen(Ag) and antibody(Ab)in sera from varioussubjects with improved method for CIC
dissociation were carried out by ELISA using polyclonal anti-body(PcAb),monoclonal
antibedy(McAb)and soluble Schistosome egg antigen(SEA ).170 caseswith various stages of
schistosomiasis(acute, chronic and advanced),cured schistosomiasis, paragonimi-asis and 65
healthy individuals were examined, The levels(mean OD values)of specific Ag and Ab
de-tected(NF一CAg and CAb)showed satisfactory specificity and sensitivity.This method was
consid-ered to be of value in the complementary diagnosis of schistosomiasis and the
evaluation of effectivechemotherapy.
出处
《中国寄生虫病防治杂志》
CSCD
1995年第4期286-289,共4页
Chinese Journal of Parasitic Disease Control
基金
国家"八五"攻关课题
关键词
血吸虫病
日本血吸虫病
多克隆抗体
单克隆抗体
Schistosomiasis,circulating immune
complexes, dissociation, soluble schistosome eggantigen,monoclonal antibody, polyclonal
aintibody,complementary diagnosis'8.5'National Key Task Project
