摘要
用马拉硫磷抗性致倦库蚊酯酶免疫经PPD刺激的小鼠制备了5株单克隆抗体,经鉴定均为IgG1,各株单抗两两相加的相加指数(A.I.)均未超过50%。酯酶与1∶1000稀释的腹水孵育1h后即失去分解底物活性。免疫印迹结果显示,单克隆抗体识别的抗原在8个群体(或品系)均有一64kDa蛋白带,反应强度及蛋白带数量与抗性程度有关。单抗对幼虫及苄呋菊酯抗性品系酯酶基本无反应。
Five clones of monoclonal antibodies (McAbs) were generated from BALB/c mice which were activized with PPD and immunized with esterases extracted from malathion resistant Culex quinquefasciatus . All 5 clones of McAbs belonged to the IgG 1 subtype, additive index (A.I.) of every two McAbs did not exceeded 50%. After a l h of incubation, the McAbs at the titer of 1∶1 000 completely inhibited the activity of the amplified esterases. Of 8 strains of adult mosquitoes studied by Western blot assay, all exhibited the 64 kDa esterase band recognized by the McAbs, six (SP,FS,SH,CD,RD,RM) showed more bands of different MW (43-60 kDa), the higher the organophosphorus resistant level, the more and the denser the bands. The above McAbs had little or no effect against the esterases of dimethrin resistant strain and the larva stage of malathion resistant Culex quinquefasciatus .
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1995年第1期42-45,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
有机磷抗性
库蚊
酯酶
单克隆抗体
制备
鉴定
Organophosphorus resistance, Culex mosquito, esterase, monoclonal antibodies