感染日本血吸虫单性尾蚴兔抗体水平的观察

OBSERVATION ON ANTIBODY LEVELS OF RABBITS INFECTED WITH SINGLE SEX CERCARIAE OF SCHISTOSOMA JAPONICUM

对９只雄性尾蚴感染兔（检虫１５－１３３条，平均５３．１条）及９只雌性尾蚴感染兔（检虫１－１０２条，平均３６．１条）定期采血，用ＥＬＩＳＡ、ＬＡ及ＣＨＲ进行检测，至感染后１年阳性率依次为８８．９％、５５．６％及８３．３％。３种方法检出阳性反应时间分别为感染后３－５１ｗｋ、４－１７ｗｋ及２－３５ｗｋ，其中ＥＬＩＳＡ法波动性较大，ＬＡ及ＣＨＲ则较稳定。此结果从一个侧面提示检测抗体的方法不应偏废，至少对于血防监测用于发现新感染是值得推荐的。

Nine rabbits infected with male S. japonicum cercariae(worm recovery 15-133,mean 53.1)and 9 rabbits infected with female cercariae(worm recvovery 1-102,mean 36.1) were bled periodically until 1 year post infection.The sera were tested by ELISA、LA and CHR for antibody detection.Results showed that the positive rates were 88.9%、55.6% and 83.3%,respectively.The duration of positive reaction was 3-51 wk post infection for ELISA,4-17 wk for LA and 2-35 wk for CHR and the fluctuated results were obtained in ELISA while positive CHR and LA results usually remained stable.The above mentioned results suggest that antibody detection methods should not be neglected due to its sensitivity although antigen detection methods are considered more favorable.For the surveillance of schistosomiasis in endemic areas where schistosomiasis is under control,antibody detection methods are still worthy of recommending for case finding of new infections.