摘要
从被EDS＇76病毒感染的鸭胚尿囊液中提取病毒核酸,经Pstl酶切后重组到PT_7/T_3a—19的PstI位点,再转化到Ecoli DH 5a中。在LB平板上筛选了一个EDS特异的PTEZ·P28重组质粒。用双脱氧末端终止法测定了该重组质粒中插入片段的一段核酸序列,在此基础上设计了一对引物,用这对引物成功地从EDS＇76—DNA上扩增出了一个209 bP的核酸片段。
EDS'76 DNA was extracted from the infected duckembryo allantoid fluid and digested with endohuclease Pstl. The produced fragments were recombinated with pT7 / T3a - 19 and transfered to E. Coli DH5a. A EDS'76 -specifically recombinated pTEZ p28 was screened from LB plates. The recombinant was sequenced by dideoxy mediated chain terminal method. A pair of primers were designed and synthesized according to recpmbinant's sequences. By using the primers, a 209 bp nucleic acid fragment was successfully amplified from EDS'76 -DNA.
关键词
产蛋下降综合症
基因扩增
鸡病
病毒
Egg Drop Syndromes (EDS'76) , DNA sequencing, gene amplification
