新免疫抑制剂─—康乐霉素C的发酵、分离、理化性质和生物学性质

A novel immunosuppressant,kanglemycin C,its fermentation,isolntion,physico-chemical and biological properties

从我所提供的Ｎｏｃａｒｄｉａｍｅｄｉｔｅｒｒａｎｅｉｖａｒ．ｋａｎｇｌｅｎｓｉｓ１７６７－６４已分离出了３种确定了经构的新抗生素，即康乐霉素Ａ［１］、康乐霉素Ｃ［２］和３１－ｈｏｍｏｒｉｆａｍｙｃｉｎＷ［３］。由于康乐霉素Ｃ具有很强的免疫抑制作用，在体外其活性如环孢菌素Ａ，具有很好的抗肿瘤活性。本文报道康乐霉素Ｃ的发酵、分离、理化性质和生物学性质。

A novel immunosuppressant, kanglemycin C was isolated from the culturefiltrate of a Nocardia strain,Nocardia mediterranei var Kanglensis 1747-64. The antibiotic wasextracted with ethyl acetate, purified by column chromatography using silica gel andSephadex LH-20 successively , and finaliy crystallized by methanol as yellow needle crystals.The molecular formular was determined to be C19H18O5(molecular weight 326). The antibi-otic was weakly active against Gram-positive bacteria and potent immunosuppressive andantitumor activities in vitro.