亚麻微生物脱胶技术的研究──Ⅱ．脱胶菌株的鉴定

STUDY ON TECHNIQUES OF MICROBIAL DEGUMMING OF FLAX Ⅱ. Identification of degumming strains

脱胶菌Ｋｅ３４和Ｌｅ１０能在３５℃有氧条件于普通琼脂（即营养琼脂）培养基上生长。早期菌落为不规则圆形，表面暗淡，不透明、粘稠、周边不整；后期菌落出现皱褶、干燥、乳白色。普通肉汤内产生有皱纹的菌膜，振之不碎散，菌液不浑浊，无沉淀。菌体均为革兰氏（Ｇｒａｍ＇ｓ）阳性，杆状，散在，大小：Ｋｅ３４为０·６－０·９×２·４－３·５μｍ，Ｌｅ１０为０．６－０．８×２．１－３．３μｍ；产生芽孢，中生或偏中生，不使菌体明显膨大，大小：Ｋｅ３４为０．５－０．８×１．０－１．６μｍ；Ｌｅ１０为０．５－０．ｇ×１．１－１．６μｍ；萄萄糖琼脂上的幼龄菌，美兰淡染着色均一；它们均能运动。生理生化：均在７％ＮａＣｌ中生长，石蕊牛奶不产酸、胨化，ｐＨＳ．７生长，Ｖ－Ｐ反应阳性，水解淀粉，硝酸盐还原为亚硝酸盐，好氧生长，不利用两酸盐等。因此对它们种的鉴定为芽孢杆菌属的枯草芽孢杆菌。

Both of the degumming strains (Ke34,Le10)could grow in nutrition -agar culture,at 35℃,and under aerial condition. Early,the colonies are round,dim,un - transparent,sticky, with dis- entire margins, late,dry, milky white color,and apparet fold. Wrinkling bacterial membrane in thebroth culture occur, isn't vibrated disbanding. The cultured bacterial liquid isn't turbid,in which there isn't sediment. The bacterid bodies are Gram - positive rods, occur singly, 0. 6 to 0. 9 by 2. 4 to, 3. Μm (Ke34)and 0. 6 to 0. 8 by2. 1 to 3. 3μm (Le10). The degumming strains could form spores that are oval or round,middle and deviating middle,don't apparently swell the cells,0. 5 to 0. 8 by 1. 0 to 1. 6 pm(Ke34)and 0. 5 to 0. 9 by 1. 1 to 1. 6pm, (Le10).There aren't the pallets dyed by toludine blue in inners of the young bacterial bodiesgrouing in glucose-agar culture. Cell of the degumming strains are motile,The physiological properties determined both of degumming strains show ther could grow in the presence of 7 % solium chloride,pH 5. 7,and oxygenis condition,make litmas milk peptonized,not produce acid. V-P rection apparet positive resolt,starch hydrated,reduce NO, to NO,and couldn't utilize propionate et al. So both of the degumming strains(Ke34,Le10)are identificated Bacillus subtilis