摘要
比较了苎麻子叶与子叶悬浮细胞两种材料的原生质体在分离和培养时的差异。取真叶初露期的绿色子叶,用3%纤维素酶、0.5%离析酶的0.6M甘露醇混合溶液处理5小时,原生质体产量可达5×10 ̄6个/gfr·wt,但其原生质体以海藻酸钠包埋方式培养在附加2,4—D0.5mg/L、KT0.5mg/LKM_8P培养基上,原生质体仅发生二次分裂;子叶悬浮细胞只有在纤维素酶4.5%,离析酶0.8%、半纤维素酶0.8%的0.55M甘露醇混合液中处理11小理,原生质体才达最大程度释放,每克悬浮细胞可得原生质体2×10 ̄6个,但其原生质体易于诱导分裂,在与前者相同的培养条件下,50天左右可形成肉眼可见的小愈伤组织,该愈伤组织经过扩增,在不同的培养基上可诱导芽、根的分化,从而形成完整植株。
The differences of isolation and culture between cotyledon protoplast and protoplast from cotyledon cutured cells of ramie were studied. The protoplasts (5×10 ̄6/g fr. wt)were isolated from green cotyledon swhen the ture leaves begin to emerge with the treatment of 3% Cellulase R-10,0. 5% Macerozyme R-10 in 0. 6 mol/L minnitol solution for 5hr. But they only divide 2 times when cultured on KM_8 Pmedium containing 2,4-D 0. 5 mg/L,KT 0. 6 mg/L in alginate embedding method. Protoplasts derived from cotyledon cultured cells can reach highest (2×10 ̄6/g cells)only treated with 4. 5% Cellulase, 0. 8% Macerozyme .0.8% Hemicellulase in 0. 55 mol/L mannitol solution for 11 hr. But they are easy to induce to divide and can form microcalli within fifty days when cultured on the former mediu. After subcultured,the calli can induce to from shoot and root and therefore complete plants were obtained.
出处
《中国麻作》
北大核心
1995年第3期1-5,共5页
China's Fiber Crops
基金
高校博士点基金
关键词
苎麻
子叶
悬浮细胞
原生质体
植株再生
Ramie
Cotyledon
Cultured cell
protoplast
Plant Regeneration