摘要
胰酶水解绵羊红细胞（ＳＲＢＣ）膜得到粗提物，经离子交换层析部分纯化后，获得组分ＴＲＦ－Ⅲ（Ｔｒｙｐｓｉｎ－ｒｅｌｅａｓｅｄｆｒａｅｔｉｏｎⅢ）具有明显抑制ＳＲＢＣ与猪外周血淋巴细胞形成Ｅ玫瑰花的活佐，花环形成率与ＴＲＦ－Ⅲ含量有明显的函数关系。电泳测定其分子量为６６ｋＤ。ＴＲＦ－Ⅲ单独作用尽管对ＰＢＭＮＣ的增殖无作用，但可增强ＰＨＡ刺激ＰＢＭＮＣ的增殖作用，ＴＲＦ－Ⅲ单独作用于ＰＢＭＮＣ可显著增强后者的促凝血活性，与正常相比，促凝率约增加３０％，与ＰＨＡ促凝率（３３％）相似。结果提示，ＴＲＦ－Ⅲ具有Ｅ受体配体促淋巴细胞激活作用。
he protein released from intact sheep erythrocytes
by incubation with trypsin was purifiedwith DEAE-cellulosei
on-exchange chromatography. The trypsin-released fraction(TRF-
Ⅲ)containd activity of bingding to T lymphocytes and produced
inhibition of E rosette formation. SDS-PAGE and nondena ture PAGE
analyses showed a single band with MW of about 18 000and 66 000
respectively in the final purified TRF-Ⅲ.In proliferation tests,the
results showedthat the combinations of TRF-Ⅲ and submitogenic
concentration of PHA significantly stimtilat-ed proliferation of
PBMNC while TRF-Ⅲ alone was nonmitogenic. Contrast with
requirementsin PBMNC proliferation experiment,TRF-Ⅲ alone in the
procoagluation experiment similar tosheep erythrocytes increased the
procoaglutation time by about 30%.These results suggest thatTRF-Ⅲ
contains the activity of E receptor liatural ligand.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第6期328-331,共4页
Chinese Journal of Immunology
基金
国家自然科学基金
