抗HLA－A2单抗重链可变区基因的克隆及其序列分析

MOLECULAR STRUCTURAL CHARACTERIZATION OF IM-MUNOGLOBULIN V_H REGIONS FROM MURINE MONOCLONALANTIBODIES AGAINST HLA-A2

从５株抗ＨＬＡ－Ａ２单抗杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术，扩增和克隆出单抗可变区重链基因，并采用双脱氧链式终止法及计算机基因文库测定和分析了其全部的氨基酸序列，对抗体的生物学特性的研究具有重要意义，为构建基因工程抗体奠定了基础。

e have characterized and determined the V region gene sequences of a series of mousemonoclonal anti-HLA-A2. Total cellulsr RNA was isolated from hybridoma cell lines producingthe five monoclonal anti-HLA-A2. Cloning and sequencing of cDNA from these antibodies. Wehave aligned them to evaiuate V region of structures homology.HO-1, HO-3, HO-4 and HO-5express V_H regions which are highly homologous to the Vn J558 gene family and use members ofthe Dsp2. 2 D region gene and utilize the Jx3. Alternatively,HO-2 is related to the Vn Q52 genefamily and expresses of the DFL16. 2 and D gene regions in conjunction with the Jx4 gene seg-ment. These date suggest that the antigen binding specificity of an immunoglobulin molecule isdetermined by the amino acid sequence of the variable region of heavy and light chaiii. The heavvchains play a major role in determining the specificities of the antibody combining sites epitoperecognition among the monoclonal antibodies may reflect sequence variability primarily withinthe CDR3 region of Vu.