杂交－杂交瘤细胞系的建立及其生物学特性

PRODUCTION AND BIOLOGICAL CHARACTERISTIC OF HY-BRID-HYBRIDOMA TO TARGET HUMAN LAK KILLING TUMORCELLS

研究了以产生抗人小细胞肺癌相关抗原和ＣＤ３双特异性抗体（Ｂｉｓｐｅｃｉｆｉｃａｎｔｉｂｏｄｙ）的杂交杂交瘤（Ｈｙｂｒｉｄ－ｈｙｂｒｉｄｏｍａ）的方法及双特异性抗体的性质。两个具有不同表型（ＨＡＴ敏感／Ｎｅｏ拮抗和ＨＡＴ拮抗／Ｎｅｏ敏感）杂交瘤细胞系，经常规方法进行融合。最后选出７个分泌双特异性抗体的杂交。杂交瘤细胞系并对其中一个克隆系细胞进行了测示研究。结果表明，该克隆系细胞培养上清能有效增强ＬＡＫ细胞对靶瘤细胞的杀伤活性，表明其具有临床应用前景。

detailed procedure is described forthe preparation hybrid hybridoma which produce bis-pecific anti-CD3 X anti-SCLC antibody.This is achieved by fusing two hybridoma cell lines thatare phenotypically distinct(HAT￣s/Neo￣r and HAT￣r/Neo￣s)and there by allow for the selection ofthe appropriate cells,We have isolated several stable hybrid hybridomas in which secreted bispe-cific antibodies that one specificity is against CD3 and one against small cell lung carcinoma-asso-ciated antigen. One of them,coded as WST-H7,showjng a qaudroma was done for the prelimi-iiary characterizations。We show that cuIture supernatant derived from WST-H7 can efficientlyinduce tumor-cells lysis by LAK cells. The results suggest that WST-H7 has a potential use astherapeutic agents for the treatment of cancer.