球形芽孢杆菌C_（3－41）菌株的晶体和芽孢的分离

Separation of Crystals and Spores from Sporulating Cell Culture of Bacillus sphaericus C_(3-41)

本文报道球形芽孢杆菌Ｃ３－４１菌株的晶体和芽孢的分离方法。采用１００μｇ／ｍｌ的溶菌酶处理培养物和４４％复方泛影葡胺离心，可使芽孢含量比例由５５～７５％提高到９０～９６％，但用优选的６号固体培养基培养时，芽孢纯度可达９８％以上。采用５０ｍｍｏｌ／Ｌ二硫苏糖醇（ＤＴＴ）处理芽孢，在－３０℃和室温下冻融及４２％复方泛影葡胺离心程序，离心管上清液中可获得高纯度的被分离的晶体。

A separation procedure of crystals and spores from sporulating cell culture of Bacillus sphaericus C3-41 was reported in this paper.Spore rate in the preparation was increased to 90-96% by lysozyme treatment and 44% meglumini diatrizoatis composita centrifugation.However,when the strain Ca-41 grown on the plates of number 6 medium,spore rate in the culture was over 98%.Very pure crystals were separated by a procedure that involves dithiothreitol (DTT)treatment,freezing and thawing of C3-41 culture as well as 42% meglumini diatrizoatis composita centrifugation.