摘要
免疫印迹检测36例大疱性类天疱疮(BP)患者血清中类天疱疮抗体(BPAb)。结果表明,32例(89%)BP血清与正常人皮肤热分离表皮的提取物中230kD、190kD、165kD、145kD和115kD多肽反应,其中16例(44%)血清与230kD和165kD多肽同时反应,7例(19%)血清单独与230kD多肽反应,9例(25%)血清单独与165kD多肽反应。结果提示,免疫印迹检测BPAb可用于BP诊断。
We
detected the bullous pemphigoid antibodies(BP-Ab)of sera from 3 6
patients with bul-lous pemphigoid (BP)and 20 control subjects(normal
volunteers,pemphigus vulgaris , epider-molysis bullosa acquisita )by
immunoblotting,32(89%)sera reacted with 230 kD,190 kD,165kD,145 kD
and 115 kD antigens from human heat-separated epidermis. The
different types ofreactivity couid be defined:(1)the presence of 230
kD aiitigen exclusively in 7(19%)sera;(2)the presence of 165 kD
antigen excltisively in 9(25%) sera;(3)labelling of the 230kD and165
kD antigen by same serum in 16(44%)sera,The results suggest that
detection of Bp-Ab byimmunoblotting is used for the diagnosis of BP.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第1期48-50,共3页
Chinese Journal of Immunology
基金
国家教委博士点基金
关键词
免疫印迹
类天疱疮
抗体
大疱性
Immunoblotting Bullous pemphigoid dntibodies