PHN大鼠肾内IC及GBM变化的立体研究

STEEROSCOPIC QUANTITATIVE MEASUREMENT ON THE CHANGES OF THE RENAL IC AND GBM IN THE RATS WITHPHN

应用抗大鼠肾小管抗原（Ｔｕｂ－Ａｇ）的抗血清免疫同种ＳＤ大鼠，成功地复制出被动型Ｈｅｙｍａｎｎ肾炎（ＰＨＮ）动物模型。此后，对ＰＨＮ大鼠肾内免疫复合物（ＩＣ）的体积密度及肾小球基底膜（ＧＢＭ）厚度进行立体定量测定，结果表明ＧＢＭ上皮侧比的形成由小到大呈逐渐累加过程，最终ＩＣ被ＧＢＭ样物包绕、取代，导致ＧＢＭ呈不规则性增厚。

he SD rats were immunized with antiserum against the renal tubular antigen(Tub-Ag)ofhomologous rats, thus, the animal model of passive Heymann nephritis(PHN)was successfullyreprodticed.And the volume density of the renal immune complex(IC)and the thickness of theglomerular basement membrane(GBM)of the rats with PHN were quan-titatively measured. The results showed that the formation of the IC on the epithelial side of GBM was gradually ac-cumulated. Finally, the IC was surrounded and replaced by basement-membrane-like meterial,which resulted in the irregular thickneing of the GBM of the rats wjth PHN.