摘要
本文研究了分泌抗天花粉蛋白ＩｇＥ抗体的同种异体小鼠杂交瘤株（Ｂａｌｂ／ｃ×Ｃ５７ＢＬ／６Ｊ）ＴＥ１的腹水的诱生，最佳诱生腹水的宿主是遗传背景相容的Ｆ１（Ｂａｌｂ／ｃ×Ｃ５７ＢＬ／６Ｊ）小鼠，其次是与杂交瘤亲本骨髓瘤细胞ＮＳ－１遗传背景一致的Ｂａｌｂ／ｃ小鼠，同时比较了各种免疫抑制处理宿主的腹水诱生率，其中以照光处理最佳，此外对杂交瘤株进行“驯化”，得到了易于诱生腹水并且抗体效价高的杂交瘤细胞。
he induction of
ascites by inoculation of mouse allogeneic hybridomas (Balb/c ×
C57BL/6J)secreting IgE antibodies to trichosanthin was studied. The
optimal hosts for ascites inductionwere mice with same genetic
backgroun, F1 (Balb/c×C57BL/6J)mice, The next was Balb/cmice,with
genetic background same to NS-1 cells. The ascites induction rate of
several methodsfor immunosuppression of the host mice were also
compared.Of them irradiation came to thefirst. Furthermore through
“training”proesses,hybridoma cells liable to induce ascites and
secret-ing high titer antibodies could be obtained.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第2期75-77,共3页
Chinese Journal of Immunology
