用PCR技术检测牙龈炎组织中IL－Iα的表达

DETECTING IL-1αEXPRESSION IN INFLAMED GINGIVA WITHPCR

为了检测牙龈炎组织中ＩＬ－１的表达，设计合成了扩增人ＩＬ－Ｉａ基因的引物，用ＲＴ－ＰＣＲ技术从牙龈炎组织ＲＮＡ中扩增了ＩＬ－Ｉα基因，而正常牙龈中扩增不到该产物。将扩增片段克隆后进行了部分序列的测定，结果表明ＰＣＲ方法可以简便，快速，特异，灵敏地检出炎症组织中ＩＬ－１的表达，这对于牙龈炎发病机理及治疗的研究具有重要意义。

his studywas designed to examine tbeexpression of IL- 1α in human inlamedgingiva. Wesynthesized a pair of primers to amplify the IL -1α gene with RT-PCR。 The results showed thatthere was IL-1α mRNA expression in inflamed gingiva but not in healthy gingiva。Partial DNAsequencing confirmed that the PCR product was human IL-1α gene,The study suggested thatPCR was a simple,fast,sensitive and specific method for the detection of IL-1α expression ingingivitis.