摘要
用纯化溶菌酶为抗原,免疫家兔制备其抗体,以及抗体的提取、纯化和鉴定,建立了一种新溶菌酶免疫测定法,并对重复性、相关性和回收试验等最佳条件进行了探讨。回收率为97%,批内CV1.98%,批间CV4.5%,与黄色微球菌琼脂平板法比较,两法呈正相关,r=0.987。溶菌酶浓度0~300μg/ml呈良好线性,正常参考值范围为9.8~40μg/ml(2s)。
his paper reported
applied purification against of lysozyme、immunologic rabbit
preparationof lysozyme antibody and Abstract purification、appraise、
etc of antibody, build a new method de-termination immunologic of
lysozyme. Also studied the repreducibility、recovery ration、
interre-lated and aptimal condition of the test.Recovery rate is
97%and within-run CV=1. 98%andbetween-run CV=4.5%,It was compared
vvith the M. lysodeikticus agar diffusion methodwith display positive
interreleted r=0. 987. Lysozyme content of 0~300μg/ml were
excellentlylinear.Normal reference range were 9.8~40μg/ml.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第5期283-285,共3页
Chinese Journal of Immunology
关键词
溶菌酶
抗体
制备
免疫学测定法
Lysozyme Diffusion Immunologic analyze[Chinese Journal of Immunology,
1995
11(5):283]