尿调素对中性粒细胞、淋巴细胞及补体功能的影响

INFLUENCE OF UrM ON THE FUNCTION OF NEUTRAL LEUKO-CYTES、LYMPHOCYTE AND COMPLEMENT

用亲和层析术从孕妇尿中提取尿调素，发现：（１）尿调素浓度＞１００ｍｇ／Ｌ时能促进中性粒细胞对白色念珠菌的吞噬作用；（２）抑制分离的Ｔ淋巴细胞的转化，但对未分离的Ｔ淋巴细胞的转化及分泌ＩＬ－２、ＩＦＮ－γ的功能无影响；（３）对ＳＲＢＣ免疫的小鼠脾细胞和分泌ＣＭＶ单抗的杂交瘤细胞分泌抗体的功能无影响；（４）对补体经典途径的激活无影响。

romodulin(UrM)is an 85kD acidic glycoprotein purified from the urine of normal preg-nant women,its genes localizes to 16P13.11. Recent studies demostrated that it can bind withcytokines and inhibit an antigen-specific human T-cell proliferation. So it is considered as a pos-sible immunosuppressant. The studies were aimed to probe into effect of UrM on polymorphonu-clear leukocytes(PMNL)、 lymphocyte and complement in vitro. Found:(1)UrM promoted thephagocytotic ability of PMNL when the concentration was more than 100mg/L;(2)UrM inhib-ited antigen-specific T-cell proliferation when the T-cell was isolated from the whole blood,oth-erwise it couldn’t affect the function of T-cell secreting IL-2、IFN-γ.(3)UrM had no effect onactivation of classic complement pathway and on antibody-secreting cells function includingspleen cells of mouse immunized with SRBC and hybridoma cells secreting anti-CMV monoclonalantibody. These results suggested UrM has no up and down regulation in vitro.