摘要
CD23特异性配体(抗CD23McAb和IgE免疫复合物)与表达CD23分子的活化8细胞共育,然后分析其对B细胞增殖和分化的影响。结果表明:抗CD23单抗及IgE免疫复合物(IgEIC)对B细胞增殖呈促进和抑制双向调节效应,即:在高浓度(CD23单抗>0。16μg/ml,IgEIC>2.5μg/ml)时呈剂量依赖性抑制,而在某一适当浓度范围内CD23McAb在(0.0025~0.05μg/ml,IgEIC在0.08~0.32μg/ml)呈促增殖效应。FACS分析观察到促B细胞增殖浓度的CD23单抗交联膜CD23分子(mCD23)可促进B细胞表面免疫球蛋白(sIg)的转型,即使IgM+/IgD+B细胞进一步向浆细胞(AFC)或记忆细胞分化。CD23McAb对B细胞增殖、分化的调节有赖于B细胞mCD23的表达。其次,适当浓度的交联使CD23McAb与其所抗不同表位结合而传递阳性信号,促使B细胞Ig重链转型。这一发现表明mCD23在B细胞增殖、分化中起重要的调节作用。
irst of all,Blymphocytes pretrated with SAC were
cultured with CD23 ligands,anti-CD23McAb and IgE immuno
complex(IgEIC)respectively.By MTT colorimetric assay,we foundthat
anti-CD23 McAb and IgEIC could significantly influence the
proliferation of CD23 ̄+ B cellsat a figure of twoway phenomenon,that
high concentration of ligands inhibited the proliferationof B cells
whereas in1proved the proliferation in a suitable concentration of
ligands. Furtherly,we selected a optimal CD23 McAb concentration(0.05
μg/ml)for crosslinking mCD23.BY FACSanalysis,the results indicated
that antiCD23 McAb could transmit B cells proliferation
signals,promote the expression of Ig in B lymphocytes,induce Ig
switching and increase the steadiness ofmembranous CD23.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第6期336-339,共4页
Chinese Journal of Immunology
基金
卫生部基金