三种方法检测沙眼衣原体感染宫颈标本的比较研究

The Comparative Study or Three Methats for Detection of Chlamydia Trachomatis Infections in Cervical Specimens

采用ＤＩＧ标记的沙眼衣原体ＤＮＡ探针斑点杂交、细胞培养和免疫荧光三种方法对５４０例高危人群宫颈标本沙眼衣原体感染进行了检测，其阳性率分别为３０．０％、２９．６２％和３０．３７％。并发现ＤＩＧ标记的ＤＮＡ探针检测标本与细胞培养法具有相似的敏感性和特异性，高于免疫荧光法。因而可作为一种简单、快速、准确的沙眼衣原体检测方法。

DIG-DNA probe dot blot,cell culture and direct immunofluorescent antibody (DIA ) assays were adopted to detect chlamydia trachomatis infections in cervical specimens of 540 cases high risk women. The results indicated the positive rates was 30. 0%, 29.62% and 30. 37 % respectively. We found the sensitivity and specificity of DNA probe dot blot were similar to that of cell culture and higher than that of DIA.This result denotes that DNA probe may be a relatively simple,rapid and accurate test.