应用PCR技术检测孕早期绒毛组织弓形虫DNA的研究

Detection of Toxoplasma gondii DNA in Choriouic Villus of Pregnant Women during the First Trimester by PCR

应用ＰＣＲ技术检测２７０例孕６～１２周妇女外周血及相应绒毛组织中的弓形虫ＤＮＡ，结果弓形虫ＤＮＡ阳性２６例，相应的绒毛组织中检出８例，垂直传播率为３０．７７％（８／２６）。同时用ＥＬＩＳＡ法检测血清中弓形虫循环抗原（ＣＡｇ）、ＩｇＭ和ＩｇＧ抗体，结果阳性率分别为３．７０％、７，０３％和１５．５％。ＩｇＭ和／或ＣＡｇ阳性者，外周血皆检出弓形虫ＤＮＡ。提示应用ＰＣＲ技术检测绒毛组织弓形虫ＤＮＡ结合ＥＬＩＳＡ进行筛选，可早期诊断先天性弓形虫感染，较之用羊水、胎儿血进行产前诊断时间提前８～１２周，有利于尽早治疗或中止妊娠。

Polymerase Chain Reaction(PCR) was used to detect Toxoplasma gondii DNA(TOXO-DNA) in meternal blood and chorionic villus.A total of270 cases of pregnant women was surveyed.Circulating Antigen(CAg)、IgM. and IgG antibodies in sera were examined by ELISA in the mean time.The result showed that:(1) 26 cases of maternal blood TOXO-DNA were positive,including 8 cases of chorionic villus TOXO-DNA,The vertical transmission rate was 30.77%(8/26).(2) The positive rate of CAg was 3.70%,IgM7.03% and IgG 15.50%.(3) In case IgM and/or CAg were positive,TOXO-DNA could be detected in maternal blood.This study offers a simple,fast and accurat way for the diagnosis of congenital toxoplasmosis in the first trimester.It provides more clinical material for patient's treatment.