抗乙肝转移因子口服液的研制

Study of Oral Preparation of HBV Transfer Factor

将乙肝疫苗采用肌肉和皮内混合免疫的方法,给绵羊接种。全程免疫 3次,6 mo后,取血及脾脏,分离白细胞,用离心和超滤的方法,提取转移因子,制成口服液,并对其所含成分、性质进行了研究。结果表明,该口服液的主要成分为核苷酸、多肽,并含有18种游离氨基酸,包括人体必需的8种氨基酸以及人体所必需的6种常量和微量元素。动物实验表明,无毒、无任何副作用。活性玫瑰花和~3H-TdR掺入法测定的淋巴细胞转化实验表明,该口服液具有转移因子注射制剂促进T淋巴细胞增殖和依赖于乙肝抗原的免疫活性,并可在不同动物间转移免疫活性。

3 sheep were immunized with Hepatitis B vaccine by intramuscular and intracutaneous inoculations 3 times. 6 months after immunization, the sheep blood and spleens were collected. The HBV Transfer Factor was extracted and isolated by ultrafiltration. The oral preparation of HBV TF was prepared according to the Chinese Requirements of Biologies. Biochemical analysis demonstrated that this preparation is composed of nucleotides, polypeptides, free aminoacids and different trace elements. The animal experiments indicated that it is nontoxic. The active Rosset formation test and the experiments of 3H-thymidine in corporation showed that this oral preparation had the same action as the TF injection