蜂毒中磷酯酶A_2和透明质酸酶的活性分析

Assays of Phospholipase A2 and Hyaluronidase in Bee Venom

运用酶学方法分析了蜂毒中两个主要大分子成分磷酯酶A_2和透明质酸酶的活性,并与部分纯化后的峰毒中上述两个酶蛋白的活性作了比较。结果发现,部分纯化后的蜂毒几乎不含透明质酸酶、磷酯酶A_2,活性由原来的180u/mg下降至16u/mg。

Phospholipase A2(E. C. 3, l,l,4)and Hyaluronidase (E. C. 4,2,2, Dare two main high molecular weight components in bee venom. We describe the methods in enzymatic analysis in compari-sion of activity of phospholipase A2 and hyaluronidase between the crude bee venom and the purified bee venom.