三种保存昆虫病原线虫共生菌的简易方法

THREE SIMPLE METHODS FOR STORAGE OF BACTERIAL SYMBIONTS OF ENTOMOPATHOGENIC NEMATODES

在室温下（１２～２８℃），用１％ＮａＣｌ溶液、蒸馏水和液体石蜡保存４种２８株昆虫病原线虫的共生菌。结果，所有３种方法都能保持共生菌初生型活力达２年以上。在１０℃、２３℃恒温下，用１％ＮａＣｌ溶液保存的共生菌，保存期亦超过２年。在４℃下，用１％ＮａＣｌ溶液保存的共生菌半数以上可达６个月。

Twenty eight strains of bacterial symbionts of entomopathogenic nematodes, belon ging to four species of Xenorhabdus and Photorhabdus , were stored with t hree methods (1% sodium chloride solution, distilled water and paraffin liquid) at room temperature (12￣28℃) and tested for their duration of maintenance. The results demonstrated that all strains of tested bacterial symbionts could k eep their primary form and antibiotic activity for two years with the three meth ods. The duration of maintenance stored with 1% sodium chloride solution was o ver two years at 10℃ and 23℃ constant temperature. More than half strains st ored with 1% sodium chloride solution could survive over 6 months and most strai ns stored with distilled water and paraffin liquid died in 6 months at 4℃.