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大麦芽根发酵培养苏云金杆菌的初步研究 被引量:2

FERMENTATIVE CULTURE OF BACILLUS THURINGIENSIS SUBSP. KURSTAKI (HD-1) BY USING " MALT SPROUT ROOTS" AS CULTURE MEDIA
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摘要 大麦芽根是啤酒工业的副产品,它含有较丰富的粗蛋白质和可溶性无氮物。采用麦芽根培养基,进行了苏云金杆菌HD-1发酵试验。结果表明,在适当的配比条件下,该菌生长良好,产晶体蛋白能力也正常。发酵液活孢子数可以达到3.7×109/mL,而原料成本只相当于对照鱼粉、玉米培养基的1/4。 As a byproduct of brewing industry, the 'malt sprout roots'are still rich in raw proteins and water soluble N-free matter. Fermentative culture of Bacillus thuringiensis subsp. karstaki (HD-1 ) was carried out by using 'malt sprout roots'as culture media. Results showed that, under proper formulation, HD-1 grew well with sufficient production of the delta-endotoxin. Viable spores had been counted as much as 3. 7 ×1O9/ml from the culture media. The cost of the media is only 1/4 that of fish meal plus corn meal.
机构地区 烟台大学生化系
出处 《中国生物防治》 CSCD 1995年第3期111-113,共3页 Chinese Journal of Biological Control
关键词 大麦芽根 苏云金杆菌 发酵培养 Bacillus thuringiensis 'malt sprout roots' fermentation culture
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参考文献2

  • 1吴继星,谢天健,陈在佴,黄炳高,林学耀.苏芸金杆菌85-10-6(S)发酵培养基的筛选[J]微生物学通报,1988(06).
  • 2J. Arcas,O. Yantorno,E. Arrarás,R. Ertola. A new medium for growth and delta-endotoxin production by Bacillus thuringiensis var. kurstaki[J] 1984,Biotechnology Letters(8):495~500

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