摘要
利用现场ＦＴＩＲ反射光谱研究了电子中继物（介体）型ＨＣＧ酶免疫电极在不同电极电位下的电位差谱及同一电位不同状态下的单光束光谱之差。讨论了ＨＣＧ（人绒毛膜促性腺素）、抗ＨＣＧ（ａｎｔｉ－ＨＣＧ）及它们的络合物在电极表面的耦联情况和红外光谱特性。对两种不同方法制备的ＨＣＧ免疫电极的谱图加以对比和解析，并初步确定了ａｎｔｉ－ＨＣＧ的特征谱峰。
A new method of in-situ FTIR reflection spectroscopy was used instudying potential difference spectra and single beam subtracti , e spectraof HCG enzyme immunoelectrode. On the surface of electrode, IR char-acteristic spectra of HCG, anti-HCG antibody and their complex werediscussed. HCG immunoelectrode was prepared in two different waysand their spectra were compared and analyzed. The characteristic bandsof anti-HCG antibody were determined preliminarily.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
1995年第2期107-112,共6页
Chinese Journal of Biomedical Engineering
基金
厦门大学固体表面物理化学国家重点实验室
关键词
免疫电极
FTIR
反射光谱
HCG
In-situ FTIR reflection spectroscopy
Human chorionic gonadot-ropin ( HCG )
Enzyme immunoelectrode
