聚己内酯微孔膜囊管化胰组织及其胰岛素释放的观察

STUDIES ON MICROPOROUS MEMBRANE FORMACROENCAPSULATED PANCREAS

本文观察了不同孔隙度聚己内酯微孔囊管的通透性，并选择适当孔隙度的微孔囊管包裹大鼠和小鼠胰组织进行体内、外培养。结果表明，聚己内酯微孔囊管能够对胰岛素分子具有良好的通透性，而对免疫球蛋白大分子具有较好的阻挡效果。体外培养显示，囊管化胰组织可对葡萄糖刺激作出较快应答，持续培养三个月，始终有胰岛素释放。体内培养２３天后仍能对葡萄糖刺激作出反应。

Microporous membranes were made using biodegradable polycaprolactone with pore-forming material. Experimental results showed that the membrane was permeable for in-sulin and at the same time formed a good barrier to IgG. The tubular membranes (2mm i. d.and 200μpm wall thickness) were filled with mouse pancreas,closed at both ends,and cul-tured in vitro. The insulin was shown released from the macroencapsulated mouse pancreasfor over 2 months and increased in secretion in response to high glucose concentration. Afterplaced in the peritoneal cavity of the pyrimidine-tetraoxide-induced diabetic rats for 23days,the macroencapsulated rat pancreas were removed and exposed to a sequence of low(100mg/dL)and high(300mg/dL) concentration of glucose in the perifusing medium at therate of 2ml/min. The insulin concentration in the perfusate increased from 2. 0±0.8μu/mlto 22.7±4.2μu/ml with in 15 minutes.