rBS-WC霍乱疫苗的动物免疫研究

Immune Efficacy of rBS-WC Cholera Vaccine in Animals

rBS与WC联合免疫比单纯免疫效果好；小鼠或家兔的免疫间隔适当缩短未见影响免疫保护力；小鼠一次大剂量口服疫苗后可获良好保护。rBS－WC抗原、吸附霍乱类毒素疫苗（上海苗）和吸附霍乱疫苗（武汉苗）经家兔肌肉免疫，武汉苗未见抗CT反应，对CT攻击无保护，对Eltor（小川）活菌攻击保护较弱，上海苗抗CT反应比rBS－WC低。家兔口服rBS－WC半年后，抗CT及杀弧菌抗体维持较高水平，显示其动物免疫的持久性。

The authors reported that the immune efficacy of combined administration of rBS and WC cholora vaccines was better than that of single administration of each vaccine. Proper shortening of the interval of immunization to mice or rabbits was not seemed to influence immunoprotection. Gnd protection effect was observed after the oral rBS-WC vaccine in high dosage was administered to mice. rBS-WC antigen, adsorbed cholera toxoid and adsorbed cholera vaccine were administered to rabbits intramuscularly. No CT antibody response or protection to challenge of CT was observed after administration with adsorbed cholera vaccine, and the protection of the vaccine to the challenge of Eltor live bacterium was weak. The CT antibody response of adsorbed cholera toxoid was lower than that of rBS-WC vaccine. The CT and vibriocidal antithey titers at the 6th month after immunization with rBSWC vaccine still kept at higher level. It indicated that rBS-WC vaccine showed good immune persistance.