Th-LAK细胞杀伤肿瘤细胞的活性及其机理的研究

Study on Killing Activity on Tumor Cells and Its Mechanism by Th-LAK Cells

在体外观察了用rIL－2激活的人胎胸腺细胞（Th－LAK细胞），对K562癌细胞（以下称靶细胞）的杀伤过程、在倒置显微镜下，可见Th－LAK细胞呈花环状围绕靶细胞，继而靶细胞内出现颗粒，后被破坏。在扫描电镜下，Th-LAK细胞和靶细胞先互相接触．然后细胞胞浆互相融合，4h时靶细胞核膜出现孔洞，微绒毛消失，靶细胞逐渐破裂死亡。在透射电镜下Th－LAK细胞和靶细胞混合培养30min后，就可见ThLAK细胞胞浆绒毛伸向靶细胞，互相融合，染色体浓缩，溶酶体可见，细胞膜不完整，形成凋落小体以至碎裂死亡。部分Th-LAK细胞中发现微管泡结构。

The killing course of rlL-2 activated Th-LAK cells in vitro on K,,2 cells was observed. Granules could be observed under inverted microscope in K562 cells being surrounded by Th-LAK in floral hoop shape, followed by destroying the K562 cells.Observation under scanning electron microscope: Th-LAK and K562 cells were connected and fused each other at the beginning, then holes were found on the nuclear membrane of K562cells after 4hrs of mixed culture ; then microvillus disappeared, and K562 cells were destroyed gradually. By transmission electron microscopy, Th-LAK cell cytoplasmic villuses were observed sticked to K562 cells and fused with them after 30min of mixed culture. Consequently chromosomes were concentrated. lysosomes appeared, but cytomembranes were not intact with apopotosic bodies formed until K562 cells were destroyed. Microtubalar vesicles were found in a part of Th-LAK cells.