肿瘤浸润淋巴细胞治疗消化道肿瘤的初步探讨

A primary study on therapy or alimentary tumor withtumor infiltrating lymptocytes.

本文初步探讨了肿瘤浸润淋巴细胞（ＴＩＬ）治疗消化系癌肿的临床疗效。从肿瘤组织中分离出力ＴＩＬ，体外经重组白细胞介素－２（ｒＩＬ－２）培养扩增，扩增后ＴＩＬ的ＣＤ细胞亚群比例升高，细胞毒活性增强。ＴＩＬ输注总量１．５×１０９～３×１０９，治疗有效者外周血Ｔ细胞亚群比例明显升高，自然杀伤细胞（ＮＫ）、淋巴因子激活的杀伤细胞（ＬＡＫ）细胞活性明显增强。５例行根治切除者随访１１～１９个月无复发，４例姑息切除者复发２例，９例未切除者总缓解率达６６．７％。证明ＴＩＬ治疗消化系肿瘤有一定价值。

he curative effect of TIL on alimentary tumor was studied. TIL were isolated from tissue of alimentarytumor, and cultuered in vitro with rIL--2 for expansion. TIL had a elevated proportion of CD subgroupand enhanced cytotoxicity after culture. TIL were infused at the total amount of 1. 5 X 109--3 X 109. In patients with good response to TIL therapy, subgroup Tcells of PBL ascended obviously and NK, LAK activities were remarkbly increased. 5 patients underwentradical operation had no recurrence for 11 -- 19 monthsof followed up; 2 of 4 cases replased after palliativeoperation; 9 cases underwent non resection had a totalremission rate of 66. 7%. TIL has been comfirmed tohas certain value of use.