胰岛素抗体的测定和临床应用

ANTI-INSULIN ANTIBODY DETERMINATION AND ITS CLINICAL APPLICATION

本文报道用ELISA检测血清中胰岛素抗体(IAb)。观察了正常对照组、糖尿病病人胰岛素治疗组和非胰岛素治疗组IAb值,分析了IAb阳性和IAb阴性者之间年龄、空腹血糖、血浆胰岛素水平、糖化血红蛋白的变化。结果显示用胰岛素治疗组血清IAb明显高于正常对照组(P<0.05)及非胰岛素治疗组(P<0.01),IAb阳性者血浆胰岛素水平低于IAb阴性者(P<0.05)。对各组患者产生IAb的机理作了讨论,提出了有高IAb者在用胰岛素治疗时应注意的有关问题。

Anti-insulin antibody (IAb) was determined by a rapid enzyme-linked immunosorbent microassay (ELISA) in 113 cases. The results showed that the IAb in 35 patients using insulin was much higher than that in 31 normal controls and 47 patients using no insulin (P<0.05, P<0.01 respectively). Serum insulin level in lAb-positive patients was much lower than that in IAb-negative ones (P<0.05). There was no correlation between IAb and serum insulin in the patients using no insulin (r=-0.344, P>0.05). The data indicated that attention should be paid to the cases using insulin and with positive IAb. And the assay is useful for checking insulin antibody.