葡萄糖生物传感器的研制

Preparation of Glucose Sensor

将葡萄糖氧化酶以共价连接法固定于醋酸纤维素制成的ｌ薄膜上，将酶膜紧贴于极谱式氧电极表面，制成可对葡萄糖进行定量测定的葡萄糖传感器。该传感器测定范围５０～８００ｍｇ／ｄｌ，响应时间１０ｓ，相关系数ｒ１０＝０．９９８，ＣＶ＜３％。

Glucose oxidase was immobilized on the cellulose acetate membrane bycovalent binding.The enzyme membrane was attached on the top of an O2-electrode toobtain a sensor specific to glucose. The assay range of the sensor was 50～80 mg/dLand CV<30%,r=0.998,the response time was 10 seconds.