琼脂扩散试验诊断兔粘液瘤病的研究

STUDIES ON THE KIAGNOSIS OF RABBIT MYXOMATOSIS USING AGAR-GEL IMMUNODIFFUSION TEST(AGID)

本试验建立了兔粘液瘤病琼脂扩散诊断方法。抗原用乙烯胺灭活，通过易感动物检测灭活效果。琼脂板制备条件：琼脂糖浓度为０．８～１％，ｐＨ７．４，ＮａＣｌ浓度为０．７～０．８５％。

AGID was estblished for the diagnosis of rabbit myxomatosis, The antigen for AGID was in-activatid by BEI and the effect of inactivation was detected by inoculating sensitive anirnals.The results of ex-periments showed that the conditions for preparation of agar-gel plates were as follows:0.8～1.O%agrose,0.7-0.85%NaCl, pH value 7.4.