栽培稻和普通野生稻基因组的随机扩增多态性DNA（RAPD）初步分析

A Primary Study of Cultivated Rice(Oryza sativa)and Common Chinese Wild Rice(O.rufipogon)Using Random Amplified Polymorphic DNA(RAPD)

用３５个随机引物对５个籼稻品种、５个粳稻品种和２７份中国普通野生稻进行了ＲＡＰＤ分析。６０％以上的引物能在籼稻和粳稻基因组间显示差异；中国普通野生稻与栽培稻的差异主要表现在与籼稻的不同，绝大部分供试野生稻的ＲＡＰＤ带型与粳稻的相同。这说明多数中国普通野生稻偏粳，但也存在偏籼的普通野生稻。

Ten cultivated rice varieties(O.sativa) and 27 accessions of Chinese common wild rice(O.rufipogon)were analyzed by RAPD using 35 primers.The results indicated that almost 60% of used primers could reveal polymorphisms between indica and japonica varieties.The difference of O.rufipogon to O.sativa disptayed mainly between the wild rice and O.sativa ssp.indica.It suggested that most accessions of wild rice have the similar RAPD patterns to that of O.sativa ssp.japonica.That indicated that most Chinese common wild rice is similar to japonica type,and also some Chinese common wild rice is similar to indica type.