不同生态型普通野生稻花药培养力的研究

Study on Anther Culture Ability of different Ecotypes of O. rufipogon Griff

对我国匍匐、倾斜和直立型普通野生稻进行了花药培养力的研究。供试的３７份普通野生稻其花药培养力存在明显差异，愈伤诱导率变幅０～９．１％，绿苗率变幅０～３２．１％，其中有３１份材料可得到花培绿苗，占８３．８％。花药培养力的高低顺序为：栽培籼稻＞栽培稻／野生稻杂种＞直立型普通野生稻＞倾斜型普通野生稻＞匍匐型普遍野生稻。不同培养基对不同生态型普遍野生稻具有不同的花培效率。改良Ｎ６－号培养基具有显著提高花培效率的作用，适合各型普通野生稻的花药培养。

Thirty-seven entries of O.rufipogon from China including three ecotypes named as stolon, slope and erectness were studied on anther culture ability. The result indicated that there existed a obvious differences in anther culture ability in the tested entries, in which the callus induction rate ranged in 0-9.1%, and green plantlet differentiation in 0-32.1%. Pollen green seedlings were obtained in 31 of the 37 entries. The anther culture ability in order was as follows: cultivated rice>interspecific hybrids>erectness ecotype>slope ecotype>stolon ecotype. different media showed different efficiency of anther culture to the tested ecotypes. However, improved N6-1 medium was the best one suitable to all three ecotypes of O.rufiporgon, which could lead to apparent increase in the efficiency of anther culture.