摘要
用潮霉素Ｂ（ｈｙｇｒｏｍｙｃｉｎＢ）抗性基因和λ噬菌体的ｃｏｓ位点组建了一种ｃｏｓｍｉｄ质粒ｐＳＶｈｙｇＢ，以此质粒建立了稻瘟病菌的转化体系，并且构建了病菌的基因组文库。结果表明，ｐＳＶｈｙｇＢ质粒可用于稳定地转化稻瘟病菌，转化频率约为１５个转化子／μｇＤＮＡ，电击法转化并不能有效地提高稻瘟病菌的转化频率。构建的稻瘟病基因文库中包含将近１００００个重组子克隆，随机挑取１２个重组子的鉴定表明，都包含有外源插入片段，说明所建文库已达要求。
A new cosmid vector pSVhygB containing hygromycin B resistance gene and bacterlophageλcos site was constructed. Using the pSVhygB the genomic DNA library of Magnaporthe grisea was constructed. It showed that pSVhygB can stably transform M. arista with frequency 15 transformants per mlcrogarn DNA and electroporation could not improved the transformation efficiency. There approximately had 10,000 recombinant clones in our genomic DNA library, more than that of the requirement for M. grisea.
出处
《中国水稻科学》
CSCD
北大核心
1995年第3期156-160,共5页
Chinese Journal of Rice Science
关键词
基因组文库
稻瘟病菌
转化
水稻
cosmid vector
genomic DNA library
hygromycin B resistant marker
Magnaporthe grisea
transformation
