实验性链脲佐菌素糖尿病动物模型的研究

A study on experimental diabetes animal models induced by streptozotocin

采用腹腔内１次注射６０ｍｇ／ｋｇ体重ＳＴＺ方法，建立了速发型链脲佐菌素Ｗｉｓｔａｒ大鼠糖尿病模型。采用每周１次连续３周腹腔内注射ＣＦＡ０．５ｍｌ和ＳＴＺ（２５ｍｇ／ｋｇ）方法，建立了迟发型Ｗｉｓｔａｒ大鼠糖尿病模型。结果提示：速发型模型建立的机制与ＳＴＺ直接损伤胰岛β细胞有关，迟发型大鼠糖尿病模型胰岛β细胞损伤可能与Ｔ淋巴细胞介导的免疫机制有关。

This paper reports the establishment of rapid and delayed diabetic experimental models in Wistar rats with streptozotocin (STZ). The rapid diabetic model was induced by 60mg/kg STZ once intraperitoneally. The rats developed classical diabetic symptoms and showed a triphasic hyperglycemia lasting for six months without recovery. Histological studies of the pancreas showed reduction of islets,degeneration of islet cells,interstitial fibrosis and hypoplasia of islet cells. The mechanism of establishing rapid diabetic model is closely related to β cells damaged directly by STZ. The delayed diabetic model was established by intraperitoneal injections of complete Freund's adjuvant (CFA) and STZ 25mg/kg once a week for 3 weeks. These diabetic rats had distinct diabetic symptoms with hyperglycemia more than 5 months without recovery and complication. Besides the above mentioned histological changes,lymphocytic infiltrations in capsules and islets of pancreas were found. This suggests the mechanism of delayed diabetic model might be associated with the thymus dependent cellular imrnune systems.