石化废水生物处理塔内生物膜不同菌系生态关系的研究（Ⅰ）

A STUDY ON THE ECOSYSTEM RELATIONSHIPS OF DIFFERENT BACTERIAL SYSTEM IN THE BIOLOGICAL MEMBRANE OF SEWERAGE PURIFICATION SYSTEM FOR PETROLEUM INDUSTRIAL CHEMICAIS

本实验以石油化工废水生物处理塔中生物膜为材料，分离得三株苯酚降解菌（Ｐ（２８）、Ｐ（２９）、Ｐ（３０））和一株石油降解菌（Ｈ２）。分别测定了它们对酚的降解和耐受能力、适宜的生长温度、ＰＨ和氯化钠浓度。证明三株酚降解菌不仅可以苯酚为唯一碳源，而且可耐受４００毫克／升浓度的苯酚。石油降解菌则最高只能耐受１０毫克／升，并且不能以苯酚为碳源。三株酚降解菌中Ｐ（２９）和石油降解茵Ｈ２菌株适宜生长温度是４０℃－５０Ｃ，属高温型菌，Ｐ（２８）和Ｐ（３０）最适宜生长温度为２５℃－３０℃，属中温型菌。但所有上述菌株的最适宜ｐＨ均为７－８，最适宜的氯化钠浓度为１－３％。经对三株酚降解菌菌胶团形成能力的测定证明，只有Ｐ（３０）菌株可形成菌胶团。经鉴定Ｐ（３０）为动胶菌属的悬丝动胶菌（Ｚｏｏｇｌｏｅａｆｉｌｉｐｅｎｄｕｌａｌ。

Three phenol degrading bacteria designated as P28,P29,P30 respectively,and a petroleum degrading bactarium as H2,were is-olated from biological membrane in sewerage purification System forpetroleum industrial chemicals.We Have tested their ability of resis-tance and degradation for phenol,optimum growth temperature,pH an-d the concentration of NaCl.It was demonstrated that three phenoldegrading bacteria could llot only utilize phenol as the sole carbonsource,bttt also resist to the concentration of phenol as high as400mg/L. While the petroleum degrading bacterium could only resistto the concentration of phenol up to 10mg/L oly,and coull not ntilizethe phenol as a carbon source. The optimum growth temperature ofphenol degrading bacterium P29 and the petroleum degrading bacterium H2 was 40-50℃,they are thermophile,the P28 and P30 are mesop-hile,their optimum growth temperature was 25-35℃。The optimum pH of all these strains was 7-8, and the optimum con-centration of NaCl Was 1-3%. The ability of perforlning zoogloesby the three phenol degrading bacteria wastested and showed that thezoogloea could been performed by strain of P30 only The strain of P30identified as the Zoogloea filipendula of Zoogloea.