全脑缺血－再灌注时催乳素生长激素与β－内啡肽的应变效应

Changes of prolactin, growth hormone and β-endor-phin activity in complete cerebral ischemia and reper-fusion

用放免分析（ＲＩＡ）法监测了急性脑缺血及再灌注条件下动物血及脑匀浆催乳素（ＰＲＬ）、生长激素（ＧＨ）及β－内啡肽（β－ＥＰ）的活性变化。发现：急性全脑缺血及缺血３０ｍｉｎ再灌注组血及脑内ＰＲＬ、ＧＨ与β－ＥＰ活性显著高于对照组和不完全脑缺血组（Ｐ均＜０．０５）。提示：血与脑内ＰＲＬ、ＧＨ及β－ＥＰ的活性变化与急性全脑缺血－再灌注的发生、发展有其内在联系，反映脑缺血、缺氧条件下中枢神经系损伤的严重程度。此外，脑匀浆ＰＲＬ、ＧＨ及β－ＥＰ含量以下丘脑最丰富，提示：下丘脑与急性全脑缺血及再灌注条件下机体神经内分泌机制紊乱密切相关。急性脑血循环障碍条件下，积极对ＰＲＬ、ＧＨ及β－ＥＰ的动态监测，对于及时到正神经内分泌机制紊乱、稳定机体内环境、减少脑血管病的并发症和提高脑血管病的整体防治水平具有实际指导意义。

We determined prolactin(PRL),growth hormone(GH)and β一endorphin(β-EP)in plasma and brainhoniogenate in 35 rabbits with acute cerebral ischemiaend reperiusion. The main findings were as follows :theconcentrations of PRL, GH and the activity of β-EP inplasma and brain homogenate of the group of completecerebral ischemia(30 minutrtes)with subsequent reper-fusion(group I)were signmcantly higher than those ofthe control group and the incomplete cerbral ischemiagroup(group Ⅱ)(P< 0.05); These increased concentra-tions in group I were more significant than those in group Ⅱ(simple complete cerebral ischemia),(P<0.05),It strongly suggested that the changes of PRL,GH and β -Ep in plasma and brain homogenate were re- lated to the emergence and evolution of acute completecerebral ischemia and reperfusion and reflected the sever-ity of injury of the central nervous system due to cerebralischemnia and anoxia. The concentrations of PRL, GHand β -EP in hypothalamus were the highest among allbrain areas,which suggests that hypothalamus is closelyrelated to the dist urbance of neuroendocrine metabolismduring acute complete cerebral ischemia and reperfusion. It is concluded that dynamic monitoring of PRL,GH andβ-EP under the circumstance of acute cerebro一vasculardisturbance is beneficial for early correcting the distur-bance of neuroendocrine metablism, for homeostasis, fordecreasing the complica tion and for the prevention andtreatment of cerebro一vascular disease.