鲩鱼胆汁中毒致多器官衰竭及其与氧自由基关系的探讨

Multiple organ failure induced by grass carp bile andthe mechanism of oxygen free radicals:experimentalstudy

用新鲜鲩鱼胆汁灌胃诱发大鼠及小鼠的多器官衰竭（ＭＯＦ），探讨其与氧自由基的关系。实验结果：新鲜鲩鱼胆汁按比重法测其重量，小鼠ＬＤ＿（５０）为４３２０ｍｇ／ｋｇ体重；大鼠按３５００～４０００ｍｇ／ｋｇ体重灌胃，１１５只大鼠染毒后２４～１２０ｈ存活的５８只中出现ＭＯＦ率为７７．５９％；小鼠染毒后２４～４８ｈ，心、肝、肾、肺、脑及血浆内丙二醛（ＭＤＡ）含量明显高于对照组；染毒后２４ｈ血液内谷胱甘肽－过氧化物酶（ＧＳＨ－Ｐｘ）活性明显高于对照组，４８ｈ降至正常；红细胞超氧化物歧化酶（ＳＯＤ）活性在４８ｈ明显低于对照组；血液内还原型谷胱甘肽（ＧＳＨ）在染毒后１～６天呈３次抛物线变化趋势，染毒后１天升高，５天达最低值，６天开始回升，并逐渐恢复至染毒前水平。提示：用新鲜鲩鱼胆汁可制造ＭＯＦ模型，ＭＯＦ发生机制可能与氧自由基增多及抗氧化物质减少有关。

The fresh bile of grass carps was perfused into thestomaches of the rats and mice to induce multple organfailure (MOF), and the mechanism of oxygen free radi-cals in MOF was studied.The results showed that the LD_(50) in mice was4320 mg/kg.b.w. of fresh bile. Of the 115 rats poisonedby fresh bile in the dose of 3500～4000 mg/kg.b. w.only 58 remained alive for 24～120 hrs.The incidence ofMOF was 77.6g%(45/58)in the living animals.Themalonic dialdehyde (MDA ) contents in the tissues of thelivers, hearts, lungs, kidneys, brains and plasma of thepoisoned mice were significantly higher at 24～48 hrs af-ter intoxication than those of the controls. The glu-tathione peroxidase acthity was increased at 24 hrs afterbeing poisoned and returned to mormal at 48 hrs. Theerythrocyte superoxide dismutase(SOD)activity wassignificantly lower at 48 hrs after grass carp bile poison-ing than the controls. The reducing glutathione level inthe whole blood of the poisoned rats showed the tenden-cy of cubic change (y=34.15+ 6.046719x-3.635025x￣2+0.410836lx￣3)1-6 days after being poisoned and be-gan to increase on the first day of poisoning. The lowestlevel was seen on the 5th day and a tendency of linearascent(y= 23.34+0.82x) appeared on the 6th day afterpoisoning, thereafter the glutathione level gradually re-turned to normal. The results indicated that a new modelof MOF induced by fresh bile of grass carps was success-fully established. The multiple organ failure appeared tobe correlated with the increase of oxygen free radicalsand decrease of antioxidants in the blood and tissues.