火焰原子吸收法测定血清中结合钙和非结合钙

Determination of Bound Calcium and Free Calcium in Serum by Flame Atomic Absorption Spectroscopy

血清样品用硫酸铵固体盐析后,过滤,滤液即为非结合钙(游离钙)溶液,采用基体匹配的方法分别测定非结合钙和总钙含量,基差值便为蛋白结合钙。该法精密度为2.6％～3.4％,回收率为95.4％～104.5％。

This article introduces a method that serum sample is sahcd out of ammonium sulfate powder, then filtered, Respec-(?) deiermination of the ealeium leaves bound caleium. Ihe method in indicate directly caleium content of different. forms When using a technique of matrix match and adding 0, (?)Domhanum to the sample.the mterference of ommonium ulfate powder and phosphate can be eliminated. Its precision (C.V%) is 2. 6%~3- 6% and the rate of recovery is 95.4% ~101.8%