嗜热脂肪杆菌芽胞恢复培养基的研究

STUDY ON THE RECOVERY CLTURE MEDIUM FOR SPORES OF Bacillus stearothermophilus

以新设计的恢复培养基、普通营养琼脂培养基及Ｆｅｅｈｅｒｒｙ报告的培养基对热损伤嗜热脂肪杆菌芽胞复苏作用进行了比较。结果，新设计的恢复培养基上的菌落明显多于另两种培养基。所试５６℃与６０℃两个培养温度，对嗜热脂肪杆菌芽胞的复苏无明显影响。

There are many factors influencing the recovery culture of spores of Bacillusstearothermophilus used for examining the efficacy of autoclaving especially in quantitativetest.The components of the recovery culture medium reported by the foreign authors werequite different,while ordinary nutrient agar was commonly used in China. The resuscitatingeffects of the recovery culture medium developed recently, the ordinary nutrient agar and theculture medium reported by Feeherry(AAMS)on the thermoinjured spores of B.stearothermophilus were compared. The results showed that the colonies on the recovery culture medium developed recentlywere significantly more numerous than those on the other 2 media. The two culture tempera-tures tested,i.e. 56℃and 60℃,had no significant influence on resuscitation of spores of B.stearothermophilus.