氯消毒对甲型肝炎病毒抗原性和感染性作用的比较

COMPARATIVE EFFECTS OF CHLORINE DISINFECTION ON ANTIGENICITY AND INFECTIVITY OF HAV

比较了氯对悬液中ＨＡＶ抗原性和感染性的作用。作用３０分钟，破坏ＨＡＶ抗原性与感染性的有效氯浓度分别为５０ｍｇ／Ｌ与２５ｍｇ／Ｌ。有机物对其消毒作用有影响。此外，对ＥＬＩＳＡ检测ＨＡＶ抗原的灵敏度进行了试验观察。

At present time,destruction of antigenicity is used most frequently as the indicator ofdisinfection of HAV. In the present study,HAV multiplied in tissue culture(the HAV usedwas NJ-3 strain and the cell strain was PLC/PRF/5.which was incubated at 32℃ for 15days)was used to observe the effects of chlorine on its antigenicity and cell infectivity.Theantigenicity of HAV was examined by ELISA test of the sample directly. For examination ofinfectivity of HAV,the sample was cultured with the cells for 15 days first.Since the culturemethod used did not produce plaque,the supernatant after centrifugation of the lysate of cul-tured cells was tested by ELISA to observe whether HAV antigen appears and its titer. Theresult of ELISA was considered as negative if the ratio of OD values of test sample to negativesample(S/N)was <2.1. The results indicated that when the HAV suspension with a titer of 1:8(ELISA) was di-luted to 10￣(-2),tne antigenicity could not be detected by ELISA method,while the result of ex-amination of HAV suspension diluted to 10￣ (-5) and multiplied in the cells was still positive. Indisinfection experiment with introducing chlorine into water,the disinfectant solution con-taining 25 mg/L available chlorine with a 30 minute contact time could cause disappearanceof infectivity of HAV,while 50 mg/L was required for destruction of its antigenicity.Whendisinfectant sulution containing 100 mg/L availabel chlorine was used for disinfection ofHAV suspension containing10% calf serum,the infectivity of HAV did not disappear until af-ter exposure for 10 minutes,while exposure for 30 minutes still could not completely destroyits antigenicity.